Microfluidic systems for rapid antibiotic susceptibility tests (ASTs) at the single-cell level
نویسندگان
چکیده
منابع مشابه
A multiplexed microfluidic platform for rapid antibiotic susceptibility testing.
Effective treatment of clinical infections is critically dependent on the ability to rapidly screen patient samples to identify antibiograms of infecting pathogens. Existing methods for antibiotic susceptibility testing suffer from several disadvantages, including long turnaround times, excess sample and reagent consumption, poor detection sensitivity, and limited combinatorial capabilities. Un...
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The widespread use of antibiotics has significantly increased the number of resistant bacteria, which has also increased the urgency of rapid bacterial detection and profiling their antibiotic response. Current clinical methods for antibiotic susceptibility testing (AST) rely on culture and require at least 16 to 24 h to conduct. Therefore, there is an urgent need for a rapid method that can te...
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The susceptibility of 76 clinical isolates of Enterobacteriaceae to ampicillin, piperacillin and gentamicin was assessed by ATP bioluminescence in a 4-h test. For most organisms tested (Escherichia, Klebsiella, Enterobacter and Serratia), there was good correlation with traditional MIC values estimated on 18-h cultures. However strains of Proteus mirabilis showed false resistance to the beta-la...
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Analysis of intracellular materials at the single-cell level presents opportunities for probing the heterogeneity of a cell population. Lysis by electroporation has been gaining popularity as a rapid method for disruption of the cell membrane and release of intracellular contents. In this report, we selectively released specific intracellular molecules for interrogation at the single-cell level...
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Establishing a successful immune response requires cell-cell interactions, where the nature of antigen presentation dictates functional outcomes. Methods to study these interactions, however, suffer from limited throughput and a lack of control over cell pairing. Here we describe a microfluidic platform that achieves high-throughput deterministic pairing of lymphocytes with a defined contact ti...
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ژورنال
عنوان ژورنال: Chemical Science
سال: 2020
ISSN: 2041-6520,2041-6539
DOI: 10.1039/d0sc01353f